Blerta Kika Brati1, Enton Noçka1, Eda Hida1
1Institute of Public Heath, Tirana, Albania.
Aim: Isolation of infectious agents frequently requires specialized media. Culture media play a pivotal role in any diagnostic microbiology laboratory. Blood agar serves as an enriched, bacterial growth medium and is used for the identification and antibiotic susceptibility testing of many bacterial pathogens. The quality and sterilization of the media depends directly upon the quality of the all elements used for their preparation. The aim of this study is to evaluate the quality of agar blood medium preparation in our laboratory during 2018-2019 for microbiological diagnose.
Methods: The blood collected from the sheep is prepared based as instruction of the protocols. During the two years in preparatory laboratory are product 15 800 ml agar bloods medium. This medium is separated in 650 petri dishes with Ø 90 ml in standard sterilization environmental condition.
Results: In our institute sheep blood is the only source in the blood agar. Over all 650 petri dishes prepared in our laboratory only in one process we were before the fact of contaminated media. We have checked all standard procedure and in fin the problem was the contamination of blood. This contamination has happened during the manipulation of the sheep.
Conclusion: Thus the sterility, homogeneity, viscosity and color of the blood should be scrupulously checked before it is used for media preparation. If the conditions for manipulating and collecting blood in sheep are not in the required standard then the prepared ground will be contaminated. Hence the quality of the blood plays an important role in the performance of the blood containing media.
Keyword: culture control, microbiological diagnose, sterility control.